Essential Biosafety Equipment 解答

BSCs are open workspaces designed to protect laboratory personnelthrough the use of directional air flow and High Efficiency Particulate Air filters.

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Key considerations for selecting appropriate PPE include

• Characteristics of the biological agent
• Type of work being carried out
• Availability of national regulations
• All of the above are correct

Essential biosafety equipment do not include

• PPE
• Autoclave
• Bench
• Biological safety cabinet

HEPA filter performance of a minimum efficiency of 99.97% should be used

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Things to avoid when using a BSC

• Use open flame
• Rapid arm movements
• Block the grills
• All of the above mentioned

Which of the following description of the autoclave is incorrect?

• Do not overload the autoclave
• Use separate autoclaves for ‘clean’ and ‘dirty’ loads
• No need to monitor autoclave sterization regularly
• Do not operate overnight

Different types of glove may be needed for different tasks or with other occupational hazards, such as heat or sharps.

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Air particles are caught by HEPA through which mechanism?

• Diffusion
• Interception
• Impaction
• All of the above are correct

PPE is used only in BSL-2,3 and 4 biological laboratories

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Gloves should always be inspected before use to check that they are intact.

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When use combinations of PPE needs to consider complementing one another

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Which description is incorrect?

• Minimum distance between BSC and door is 1.1 m
• Minimum distance between 2 side-by-side BSCs is 1 m
• 2 BSCs put in the opposite site along the wall, the minimum space between 2 persons is 3.1 meters
• The airflow curtain that helps provide protection when working in a BSC is fragile

Work surfaces and interior walls need to be disinfected only after each use

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The minimum space between BSC and door is 1.1 meter and the same distance between 2 side-by side BSCs.

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Which level of biosafety laboratory required to use PPE?

• BSL-1
• BSL-2
• BSL-3
• All of the above are correct

Autoclave sterilization condiction?

• 121°C for 60 minutes
• 100°C for 60 minutes
• 85°C for 60 minutes
• 121°C for 15 minutes

UV light is not recommended to be used in BSCs

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If an autoclave used for sterilize the infectious substances, Its exhaust should be filtered through a filter with membrane size of

• 0.1 micron
• 0.2 micron
• 0.3 micron
• 0.4 micron

Autoclaving is the most dependable method of sterilizing laboratory equipment and decontaminating biohazard wastes.

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Which form of PPE is one of the most critical and easily implemented ?

• Footware
• Eye protection
• Lab coat
• Respiratory protection

After moving a BSC within the laboratory, it is not nessersary to certified the BSC.

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Which class of BSC can not protect ptoducts?

• Class III
• Class I
• Class II A1
• Class II B1

When infectious materials are sterilized, exhaust of an autoclave should be filtered through a filter with 0.2 micron membrane

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Which class of BSC do not have HEPA filtered supply air?

• Class II
• ClassIII
• Class I
• Class II B1

The proper type of PPE must be chosen and personnel must be trained in its proper use and know how to detect any faults

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Which one is not a key consideration for selecting PPE?

• Volumes and concentrations of the biological agent
• Color
• Other PPE being worn
• Individual needs of the laboratory personnel

Which of the following descriptions of the functions of a biological safety cabinet is incorrect?

• Air in the Class I, Class II B2, and Class III BSC is 100% exhausted
• There are 3 classes of BSC
• None of BSC can be applied to handle volatile toxic chemicals
• Class II A1 and A2 BSCs can be a thimble connected

The laboratory director should ensure that the adequate equipment is provided and used properly.

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Which PPE is not a core requirement?

• Respiratory protection
• Gloves
• Lab coat
• Footware

All classes of BSC can protect personnel, enviroment and product

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